Construct: shRNA TRCN0000341371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028079.2-2488s21c1
DNA Barcode:
GATGGTGTGAACTGGTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010111I01Rik (72061)
Gene Description:
RIKEN cDNA 2010111I01 gene
Transcript:
RefSeq NM_028079.2 (NON-CURRENT)
Match location:
Position 2488 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTGTGAACTGGTTATTA
Hairpin Sequence:
5'-CCGG-GATGGTGTGAACTGGTTATTA-CTCGAG-TAATAACCAGTTCACACCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTGTGAACTGGTTATTACTCGAGTAATAACCAGTTCACACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTGTGAACTGGTTATTACTCGAGTAATAACCAGTTCACACCATC-3'

Other clones with same target sequence:

(none)