Construct: shRNA TRCN0000341380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162917.1-6475s21c1
DNA Barcode:
TTCGCCATGAAGTTCTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dennd4a (102442)
Gene Description:
DENN/MADD domain containing 4A
Transcript:
RefSeq NM_001162917.1 (CURRENT)
Match location:
Position 6475 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGCCATGAAGTTCTATTAT
Hairpin Sequence:
5'-CCGG-TTCGCCATGAAGTTCTATTAT-CTCGAG-ATAATAGAACTTCATGGCGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGCCATGAAGTTCTATTATCTCGAGATAATAGAACTTCATGGCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGCCATGAAGTTCTATTATCTCGAGATAATAGAACTTCATGGCGAA-3'

Other clones with same target sequence:

(none)