Construct: shRNA TRCN0000341542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177562.4-581s21c1
DNA Barcode:
CGAGGAGATCATGGCGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec16a (74374)
Gene Description:
C-type lectin domain family 16, member A
Transcript:
RefSeq NM_177562.4 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGAGATCATGGCGTATTA
Hairpin Sequence:
5'-CCGG-CGAGGAGATCATGGCGTATTA-CTCGAG-TAATACGCCATGATCTCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGAGATCATGGCGTATTACTCGAGTAATACGCCATGATCTCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGAGATCATGGCGTATTACTCGAGTAATACGCCATGATCTCCTCG-3'

Other clones with same target sequence:

  1. TRCN0000195826