Construct: shRNA TRCN0000341552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028614.2-1214s21c1
DNA Barcode:
GTGTCCAGAAGTTCGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r1b (73699)
Gene Description:
protein phosphatase 2, regulatory subunit A, beta
Transcript:
RefSeq NM_028614.2 (NON-CURRENT)
Match location:
Position 1214 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCCAGAAGTTCGTTTAAA
Hairpin Sequence:
5'-CCGG-GTGTCCAGAAGTTCGTTTAAA-CTCGAG-TTTAAACGAACTTCTGGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCCAGAAGTTCGTTTAAACTCGAGTTTAAACGAACTTCTGGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCCAGAAGTTCGTTTAAACTCGAGTTTAAACGAACTTCTGGACAC-3'

Other clones with same target sequence:

(none)