Construct: shRNA TRCN0000341673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011507.2-499s21c1
DNA Barcode:
CCCTGGATATTTCTAGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Suclg2 (20917)
Gene Description:
succinate-Coenzyme A ligase, GDP-forming, beta subunit
Transcript:
RefSeq NM_011507.2 (NON-CURRENT)
Match location:
Position 499 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGATATTTCTAGAGAAA
Hairpin Sequence:
5'-CCGG-CCCTGGATATTTCTAGAGAAA-CTCGAG-TTTCTCTAGAAATATCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGATATTTCTAGAGAAACTCGAGTTTCTCTAGAAATATCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGATATTTCTAGAGAAACTCGAGTTTCTCTAGAAATATCCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000120823