Construct: shRNA TRCN0000341706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027613.1-3948s21c1
DNA Barcode:
CATCAGCTCTGTTGTACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mmrn1 (70945)
Gene Description:
multimerin 1
Transcript:
RefSeq NM_027613.1 (CURRENT)
Match location:
Position 3948 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAGCTCTGTTGTACTATT
Hairpin Sequence:
5'-CCGG-CATCAGCTCTGTTGTACTATT-CTCGAG-AATAGTACAACAGAGCTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAGCTCTGTTGTACTATTCTCGAGAATAGTACAACAGAGCTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAGCTCTGTTGTACTATTCTCGAGAATAGTACAACAGAGCTGATG-3'

Other clones with same target sequence:

(none)