Construct: shRNA TRCN0000341849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027384.1-3391s21c1
DNA Barcode:
CTAGCTATAGAGTATAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tet1 (52463)
Gene Description:
tet methylcytosine dioxygenase 1
Transcript:
RefSeq NM_027384.1 (CURRENT)
Match location:
Position 3391 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGCTATAGAGTATAGTAAA
Hairpin Sequence:
5'-CCGG-CTAGCTATAGAGTATAGTAAA-CTCGAG-TTTACTATACTCTATAGCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGCTATAGAGTATAGTAAACTCGAGTTTACTATACTCTATAGCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGCTATAGAGTATAGTAAACTCGAGTTTACTATACTCTATAGCTAG-3'

Other clones with same target sequence:

(none)