Construct: shRNA TRCN0000341896
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027526.1-1789s21c1
- DNA Barcode:
- GTAACCATGGTAACATCTTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rasgef1a (70727)
- Gene Description:
- RasGEF domain family, member 1A
- Transcript:
- RefSeq NM_027526.1 (CURRENT)
- Match location:
- Position 1789 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000341896)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTAACCATGGTAACATCTTAT
- Hairpin Sequence:
-
5'-CCGG-GTAACCATGGTAACATCTTAT-CTCGAG-ATAAGATGTTACCATGGTTAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTAACCATGGTAACATCTTATCTCGAGATAAGATGTTACCATGGTTACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTAACCATGGTAACATCTTATCTCGAGATAAGATGTTACCATGGTTAC-3'
Other clones with same target sequence:
(none)
