Construct: shRNA TRCN0000341900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027526.1-1281s21c1
DNA Barcode:
CCTACCCAATGGGCACATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasgef1a (70727)
Gene Description:
RasGEF domain family, member 1A
Transcript:
RefSeq NM_027526.1 (CURRENT)
Match location:
Position 1281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCCAATGGGCACATTAA
Hairpin Sequence:
5'-CCGG-CCTACCCAATGGGCACATTAA-CTCGAG-TTAATGTGCCCATTGGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCCAATGGGCACATTAACTCGAGTTAATGTGCCCATTGGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCCAATGGGCACATTAACTCGAGTTAATGTGCCCATTGGGTAGG-3'

Other clones with same target sequence:

(none)