Construct: shRNA TRCN0000341967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029152.1-406s21c1
DNA Barcode:
ACGTGGCATTTCCCTACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab10 (75040)
Gene Description:
EF-hand calcium binding domain 10
Transcript:
RefSeq NM_029152.1 (CURRENT)
Match location:
Position 406 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGGCATTTCCCTACTTTA
Hairpin Sequence:
5'-CCGG-ACGTGGCATTTCCCTACTTTA-CTCGAG-TAAAGTAGGGAAATGCCACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGGCATTTCCCTACTTTACTCGAGTAAAGTAGGGAAATGCCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGGCATTTCCCTACTTTACTCGAGTAAAGTAGGGAAATGCCACGT-3'

Other clones with same target sequence:

(none)