Construct: shRNA TRCN0000341983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033155.1-840s21c1
DNA Barcode:
TCCCGAAGACCTGTTCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajb14 (70604)
Gene Description:
DnaJ heat shock protein family (Hsp40) member B14
Transcript:
RefSeq NM_001033155.1 (CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCGAAGACCTGTTCAATAT
Hairpin Sequence:
5'-CCGG-TCCCGAAGACCTGTTCAATAT-CTCGAG-ATATTGAACAGGTCTTCGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCGAAGACCTGTTCAATATCTCGAGATATTGAACAGGTCTTCGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCGAAGACCTGTTCAATATCTCGAGATATTGAACAGGTCTTCGGGA-3'

Other clones with same target sequence:

(none)