Construct: shRNA TRCN0000342009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029430.1-431s21c1
DNA Barcode:
TGCTGACCTGGAAGCGAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc23l (75777)
Gene Description:
tetratricopeptide repeat domain 23-like
Transcript:
RefSeq NM_029430.1 (CURRENT)
Match location:
Position 431 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGACCTGGAAGCGAAATA
Hairpin Sequence:
5'-CCGG-TGCTGACCTGGAAGCGAAATA-CTCGAG-TATTTCGCTTCCAGGTCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGACCTGGAAGCGAAATACTCGAGTATTTCGCTTCCAGGTCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGACCTGGAAGCGAAATACTCGAGTATTTCGCTTCCAGGTCAGCA-3'

Other clones with same target sequence:

(none)