Construct: shRNA TRCN0000342049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029298.1-260s21c1
DNA Barcode:
TTACTGAAGAAGCCCTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stpg4 (75467)
Gene Description:
sperm tail PG rich repeat containing 4
Transcript:
RefSeq NM_029298.1 (CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACTGAAGAAGCCCTATTAA
Hairpin Sequence:
5'-CCGG-TTACTGAAGAAGCCCTATTAA-CTCGAG-TTAATAGGGCTTCTTCAGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACTGAAGAAGCCCTATTAACTCGAGTTAATAGGGCTTCTTCAGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACTGAAGAAGCCCTATTAACTCGAGTTAATAGGGCTTCTTCAGTAA-3'

Other clones with same target sequence:

(none)