Construct: shRNA TRCN0000342075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028708.1-1870s21c1
DNA Barcode:
CAAGCACTGTATCGACGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jakmip3 (74004)
Gene Description:
janus kinase and microtubule interacting protein 3
Transcript:
RefSeq NM_028708.1 (NON-CURRENT)
Match location:
Position 1870 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCACTGTATCGACGAAAT
Hairpin Sequence:
5'-CCGG-CAAGCACTGTATCGACGAAAT-CTCGAG-ATTTCGTCGATACAGTGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCACTGTATCGACGAAATCTCGAGATTTCGTCGATACAGTGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCACTGTATCGACGAAATCTCGAGATTTCGTCGATACAGTGCTTG-3'

Other clones with same target sequence:

(none)