Construct: shRNA TRCN0000342144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001144855.1-1916s21c1
DNA Barcode:
CAGGTCTGCATCGACGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppfia4 (68507)
Gene Description:
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Transcript:
RefSeq NM_001144855.1 (CURRENT)
Match location:
Position 1916 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGTCTGCATCGACGCTATT
Hairpin Sequence:
5'-CCGG-CAGGTCTGCATCGACGCTATT-CTCGAG-AATAGCGTCGATGCAGACCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGTCTGCATCGACGCTATTCTCGAGAATAGCGTCGATGCAGACCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGTCTGCATCGACGCTATTCTCGAGAATAGCGTCGATGCAGACCTG-3'

Other clones with same target sequence:

(none)