Construct: shRNA TRCN0000342248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016457.4-2053s21c1
DNA Barcode:
CACGACCAACAGATACTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKD2 (25865)
Gene Description:
protein kinase D2
Transcript:
RefSeq NM_016457.4 (CURRENT)
Match location:
Position 2053 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGACCAACAGATACTATAA
Hairpin Sequence:
5'-CCGG-CACGACCAACAGATACTATAA-CTCGAG-TTATAGTATCTGTTGGTCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGACCAACAGATACTATAACTCGAGTTATAGTATCTGTTGGTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGACCAACAGATACTATAACTCGAGTTATAGTATCTGTTGGTCGTG-3'

Other clones with same target sequence:

  1. TRCN0000001950