Construct: shRNA TRCN0000342261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000546.4-1619s21c1
DNA Barcode:
GAGGGATGTTTGGGAGATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53 (7157)
Gene Description:
tumor protein p53
Transcript:
RefSeq NM_000546.4 (NON-CURRENT)
Match location:
Position 1619 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGATGTTTGGGAGATGTA
Hairpin Sequence:
5'-CCGG-GAGGGATGTTTGGGAGATGTA-CTCGAG-TACATCTCCCAAACATCCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGATGTTTGGGAGATGTACTCGAGTACATCTCCCAAACATCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGATGTTTGGGAGATGTACTCGAGTACATCTCCCAAACATCCCTC-3'

Other clones with same target sequence:

  1. TRCN0000010814