Construct: shRNA TRCN0000342284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017572.3-475s21c1
DNA Barcode:
GCAGGTTTGAAGACGTCTACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKNK2 (2872)
Gene Description:
MAPK interacting serine/threonine kinase 2
Transcript:
RefSeq NM_017572.3 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTTTGAAGACGTCTACC
Hairpin Sequence:
5'-CCGG-GCAGGTTTGAAGACGTCTACC-CTCGAG-GGTAGACGTCTTCAAACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTTTGAAGACGTCTACCCTCGAGGGTAGACGTCTTCAAACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTTTGAAGACGTCTACCCTCGAGGGTAGACGTCTTCAAACCTGC-3'

Other clones with same target sequence:

  1. TRCN0000199614