Construct: shRNA TRCN0000342336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006296.4-616s21c1
DNA Barcode:
GGGAAGAAGTTACAGATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VRK2 (7444)
Gene Description:
VRK serine/threonine kinase 2
Transcript:
RefSeq NM_006296.4 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAAGAAGTTACAGATTTAT
Hairpin Sequence:
5'-CCGG-GGGAAGAAGTTACAGATTTAT-CTCGAG-ATAAATCTGTAACTTCTTCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAAGAAGTTACAGATTTATCTCGAGATAAATCTGTAACTTCTTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAAGAAGTTACAGATTTATCTCGAGATAAATCTGTAACTTCTTCCC-3'

Other clones with same target sequence:

  1. TRCN0000010206