Construct: shRNA TRCN0000342368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020680.3-201s21c1
DNA Barcode:
CCCGTGTCCATCTTCGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL1 (57410)
Gene Description:
SCY1 like pseudokinase 1
Transcript:
RefSeq NM_020680.3 (NON-CURRENT)
Match location:
Position 201 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTGTCCATCTTCGTCTAT
Hairpin Sequence:
5'-CCGG-CCCGTGTCCATCTTCGTCTAT-CTCGAG-ATAGACGAAGATGGACACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTGTCCATCTTCGTCTATCTCGAGATAGACGAAGATGGACACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTGTCCATCTTCGTCTATCTCGAGATAGACGAAGATGGACACGGG-3'

Other clones with same target sequence:

  1. TRCN0000007126