Construct: shRNA TRCN0000342369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003718.3-4375s21c1
DNA Barcode:
CGACGTAGTTTCATTGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK13 (8621)
Gene Description:
cyclin dependent kinase 13
Transcript:
RefSeq NM_003718.3 (NON-CURRENT)
Match location:
Position 4375 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGTAGTTTCATTGGAAAT
Hairpin Sequence:
5'-CCGG-CGACGTAGTTTCATTGGAAAT-CTCGAG-ATTTCCAATGAAACTACGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGTAGTTTCATTGGAAATCTCGAGATTTCCAATGAAACTACGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGTAGTTTCATTGGAAATCTCGAGATTTCCAATGAAACTACGTCG-3'

Other clones with same target sequence:

  1. TRCN0000194804