Construct: shRNA TRCN0000342448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003576.3-882s21c1
DNA Barcode:
CCCTGGGCATAACAGCTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK24 (8428)
Gene Description:
serine/threonine kinase 24
Transcript:
RefSeq NM_003576.3 (NON-CURRENT)
Match location:
Position 882 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGGCATAACAGCTATTG
Hairpin Sequence:
5'-CCGG-CCCTGGGCATAACAGCTATTG-CTCGAG-CAATAGCTGTTATGCCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGGCATAACAGCTATTGCTCGAGCAATAGCTGTTATGCCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGGCATAACAGCTATTGCTCGAGCAATAGCTGTTATGCCCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000199367