Construct: shRNA TRCN0000342508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001892.4-1894s21c1
DNA Barcode:
GCCACAGTTGTGATGGTTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1A1 (1452)
Gene Description:
casein kinase 1 alpha 1
Transcript:
RefSeq NM_001892.4 (NON-CURRENT)
Match location:
Position 1894 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAGTTGTGATGGTTGTT
Hairpin Sequence:
5'-CCGG-GCCACAGTTGTGATGGTTGTT-CTCGAG-AACAACCATCACAACTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAGTTGTGATGGTTGTTCTCGAGAACAACCATCACAACTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAGTTGTGATGGTTGTTCTCGAGAACAACCATCACAACTGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000199710