Construct: shRNA TRCN0000342525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005255.2-2069s21c1
DNA Barcode:
GAAGATGGCAAAGCGGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAK (2580)
Gene Description:
cyclin G associated kinase
Transcript:
RefSeq NM_005255.2 (NON-CURRENT)
Match location:
Position 2069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGGCAAAGCGGTGATT
Hairpin Sequence:
5'-CCGG-GAAGATGGCAAAGCGGTGATT-CTCGAG-AATCACCGCTTTGCCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGGCAAAGCGGTGATTCTCGAGAATCACCGCTTTGCCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGGCAAAGCGGTGATTCTCGAGAATCACCGCTTTGCCATCTTC-3'

Other clones with same target sequence:

  1. TRCN0000002156