Construct: shRNA TRCN0000342528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000082.3-263s21c1
DNA Barcode:
GATGGTGTGATTGTACTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERCC8 (1161)
Gene Description:
ERCC excision repair 8, CSA ubiquitin ligase complex subunit
Transcript:
RefSeq NM_000082.3 (CURRENT)
Match location:
Position 263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTGTGATTGTACTTTAT
Hairpin Sequence:
5'-CCGG-GATGGTGTGATTGTACTTTAT-CTCGAG-ATAAAGTACAATCACACCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTGTGATTGTACTTTATCTCGAGATAAAGTACAATCACACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTGTGATTGTACTTTATCTCGAGATAAAGTACAATCACACCATC-3'

Other clones with same target sequence:

  1. TRCN0000003721