Construct: shRNA TRCN0000342628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020397.2-1011s21c1
DNA Barcode:
TGATGGAGAAGGACCCGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Gene Description:
calcium/calmodulin dependent protein kinase ID
Transcript:
RefSeq NM_020397.2 (NON-CURRENT)
Match location:
Position 1011 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGGAGAAGGACCCGAATA
Hairpin Sequence:
5'-CCGG-TGATGGAGAAGGACCCGAATA-CTCGAG-TATTCGGGTCCTTCTCCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGGAGAAGGACCCGAATACTCGAGTATTCGGGTCCTTCTCCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGGAGAAGGACCCGAATACTCGAGTATTCGGGTCCTTCTCCATCA-3'

Other clones with same target sequence:

  1. TRCN0000001756