Construct: shRNA TRCN0000342630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001203.2-1645s21c1
DNA Barcode:
GAGCAGTGATGAGTGTCTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPR1B (658)
Gene Description:
bone morphogenetic protein receptor type 1B
Transcript:
RefSeq NM_001203.2 (NON-CURRENT)
Match location:
Position 1645 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGTGATGAGTGTCTAAG
Hairpin Sequence:
5'-CCGG-GAGCAGTGATGAGTGTCTAAG-CTCGAG-CTTAGACACTCATCACTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGTGATGAGTGTCTAAGCTCGAGCTTAGACACTCATCACTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGTGATGAGTGTCTAAGCTCGAGCTTAGACACTCATCACTGCTC-3'

Other clones with same target sequence:

  1. TRCN0000199481