Construct: shRNA TRCN0000342643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003565.2-3345s21c1
DNA Barcode:
ACATCGAGAACGTCACCAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ULK1 (8408)
Gene Description:
unc-51 like autophagy activating kinase 1
Transcript:
RefSeq NM_003565.2 (NON-CURRENT)
Match location:
Position 3345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATCGAGAACGTCACCAAGT
Hairpin Sequence:
5'-CCGG-ACATCGAGAACGTCACCAAGT-CTCGAG-ACTTGGTGACGTTCTCGATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATCGAGAACGTCACCAAGTCTCGAGACTTGGTGACGTTCTCGATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATCGAGAACGTCACCAAGTCTCGAGACTTGGTGACGTTCTCGATGT-3'

Other clones with same target sequence:

  1. TRCN0000000838