Construct: shRNA TRCN0000342644

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021005.3-1761s21c1
DNA Barcode:
CGGATATATTTCCCTGCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR2F2 (7026)
Gene Description:
nuclear receptor subfamily 2 group F member 2
Transcript:
RefSeq NM_021005.3 (NON-CURRENT)
Match location:
Position 1761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342644)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATATATTTCCCTGCTGTT
Hairpin Sequence:
5'-CCGG-CGGATATATTTCCCTGCTGTT-CTCGAG-AACAGCAGGGAAATATATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATATATTTCCCTGCTGTTCTCGAGAACAGCAGGGAAATATATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATATATTTCCCTGCTGTTCTCGAGAACAGCAGGGAAATATATCCG-3'

Other clones with same target sequence:

  1. TRCN0000005099