Construct: shRNA TRCN0000342654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001569.3-2047s21c1
DNA Barcode:
GCCACCGCAGATTATCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Gene Description:
interleukin 1 receptor associated kinase 1
Transcript:
RefSeq NM_001569.3 (NON-CURRENT)
Match location:
Position 2047 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCGCAGATTATCATCAA
Hairpin Sequence:
5'-CCGG-GCCACCGCAGATTATCATCAA-CTCGAG-TTGATGATAATCTGCGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCGCAGATTATCATCAACTCGAGTTGATGATAATCTGCGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCGCAGATTATCATCAACTCGAGTTGATGATAATCTGCGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000121138