Construct: shRNA TRCN0000342773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002637.3-4050s21c1
DNA Barcode:
GTTATTGCCTAATCACTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKA1 (5255)
Gene Description:
phosphorylase kinase regulatory subunit alpha 1
Transcript:
RefSeq NM_002637.3 (NON-CURRENT)
Match location:
Position 4050 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTATTGCCTAATCACTCCAA
Hairpin Sequence:
5'-CCGG-GTTATTGCCTAATCACTCCAA-CTCGAG-TTGGAGTGATTAGGCAATAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTATTGCCTAATCACTCCAACTCGAGTTGGAGTGATTAGGCAATAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTATTGCCTAATCACTCCAACTCGAGTTGGAGTGATTAGGCAATAAC-3'

Other clones with same target sequence:

  1. TRCN0000196578