Construct: shRNA TRCN0000342799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003617.2-833s21c1
DNA Barcode:
CCTCCATAATAACCCTGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS5 (8490)
Gene Description:
regulator of G protein signaling 5
Transcript:
RefSeq NM_003617.2 (NON-CURRENT)
Match location:
Position 833 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCATAATAACCCTGCATT
Hairpin Sequence:
5'-CCGG-CCTCCATAATAACCCTGCATT-CTCGAG-AATGCAGGGTTATTATGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCATAATAACCCTGCATTCTCGAGAATGCAGGGTTATTATGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCATAATAACCCTGCATTCTCGAGAATGCAGGGTTATTATGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000014313