Construct: shRNA TRCN0000342818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005962.4-1455s21c1
DNA Barcode:
GCTCATTTCATGCTCTGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MXI1 (4601)
Gene Description:
MAX interactor 1, dimerization protein
Transcript:
RefSeq NM_005962.4 (NON-CURRENT)
Match location:
Position 1455 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATTTCATGCTCTGCAAA
Hairpin Sequence:
5'-CCGG-GCTCATTTCATGCTCTGCAAA-CTCGAG-TTTGCAGAGCATGAAATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATTTCATGCTCTGCAAACTCGAGTTTGCAGAGCATGAAATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATTTCATGCTCTGCAAACTCGAGTTTGCAGAGCATGAAATGAGC-3'

Other clones with same target sequence:

  1. TRCN0000020474