Construct: shRNA TRCN0000342955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001747.2-620s21c1
DNA Barcode:
GCATTTCACAAGACCTCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPG (822)
Gene Description:
capping actin protein, gelsolin like
Transcript:
RefSeq NM_001747.2 (NON-CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTTCACAAGACCTCCACA
Hairpin Sequence:
5'-CCGG-GCATTTCACAAGACCTCCACA-CTCGAG-TGTGGAGGTCTTGTGAAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTTCACAAGACCTCCACACTCGAGTGTGGAGGTCTTGTGAAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTTCACAAGACCTCCACACTCGAGTGTGGAGGTCTTGTGAAATGC-3'

Other clones with same target sequence:

  1. TRCN0000029463