Construct: shRNA TRCN0000343136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018447.1-318s21c1
DNA Barcode:
GAACAAGTATCTGACAGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EMC3 (55831)
Gene Description:
ER membrane protein complex subunit 3
Transcript:
RefSeq NM_018447.1 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAAGTATCTGACAGTCAA
Hairpin Sequence:
5'-CCGG-GAACAAGTATCTGACAGTCAA-CTCGAG-TTGACTGTCAGATACTTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAAGTATCTGACAGTCAACTCGAGTTGACTGTCAGATACTTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAAGTATCTGACAGTCAACTCGAGTTGACTGTCAGATACTTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000135997