Construct: shRNA TRCN0000343186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007269.2-2143s21c1
DNA Barcode:
GCCACCTATGTACATACTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STXBP3 (6814)
Gene Description:
syntaxin binding protein 3
Transcript:
RefSeq NM_007269.2 (NON-CURRENT)
Match location:
Position 2143 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTATGTACATACTAAT
Hairpin Sequence:
5'-CCGG-GCCACCTATGTACATACTAAT-CTCGAG-ATTAGTATGTACATAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTATGTACATACTAATCTCGAGATTAGTATGTACATAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTATGTACATACTAATCTCGAGATTAGTATGTACATAGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000159454