Construct: shRNA TRCN0000343264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017785.4-2230s21c1
DNA Barcode:
CCTTTGACCAAGTGTTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPDL1 (54908)
Gene Description:
spindle apparatus coiled-coil protein 1
Transcript:
RefSeq NM_017785.4 (NON-CURRENT)
Match location:
Position 2230 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGACCAAGTGTTCAGAA
Hairpin Sequence:
5'-CCGG-CCTTTGACCAAGTGTTCAGAA-CTCGAG-TTCTGAACACTTGGTCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGACCAAGTGTTCAGAACTCGAGTTCTGAACACTTGGTCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGACCAAGTGTTCAGAACTCGAGTTCTGAACACTTGGTCAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000161450