Construct: shRNA TRCN0000343290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039500.2-3819s21c1
DNA Barcode:
TCTGCTATAACCCGAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VWA5B1 (127731)
Gene Description:
von Willebrand factor A domain containing 5B1
Transcript:
RefSeq NM_001039500.2 (NON-CURRENT)
Match location:
Position 3819 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCTATAACCCGAATTATG
Hairpin Sequence:
5'-CCGG-TCTGCTATAACCCGAATTATG-CTCGAG-CATAATTCGGGTTATAGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCTATAACCCGAATTATGCTCGAGCATAATTCGGGTTATAGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCTATAACCCGAATTATGCTCGAGCATAATTCGGGTTATAGCAGA-3'

Other clones with same target sequence:

(none)