Construct: shRNA TRCN0000343301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016099.2-267s21c1
DNA Barcode:
GTCATATCTCGAAGGTTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLGA7 (51125)
Gene Description:
golgin A7
Transcript:
RefSeq NM_016099.2 (CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATATCTCGAAGGTTGTTT
Hairpin Sequence:
5'-CCGG-GTCATATCTCGAAGGTTGTTT-CTCGAG-AAACAACCTTCGAGATATGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATATCTCGAAGGTTGTTTCTCGAGAAACAACCTTCGAGATATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATATCTCGAAGGTTGTTTCTCGAGAAACAACCTTCGAGATATGAC-3'

Other clones with same target sequence:

  1. TRCN0000134945