Construct: shRNA TRCN0000343350
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032042.5-740s21c1
- DNA Barcode:
- GCACACAGATACCGTTTATTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FAM172A (83989)
- Gene Description:
- family with sequence similarity 172 member A
- Transcript:
- RefSeq NM_032042.5 (NON-CURRENT)
- Match location:
- Position 740 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000343350)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACACAGATACCGTTTATTA
- Hairpin Sequence:
-
5'-CCGG-GCACACAGATACCGTTTATTA-CTCGAG-TAATAAACGGTATCTGTGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACACAGATACCGTTTATTACTCGAGTAATAAACGGTATCTGTGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACACAGATACCGTTTATTACTCGAGTAATAAACGGTATCTGTGTGC-3'
Other clones with same target sequence:
-
TRCN0000147451
