Construct: shRNA TRCN0000343398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007357.2-2512s21c1
DNA Barcode:
GCGTCTTCTCTCAGCGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COG2 (22796)
Gene Description:
component of oligomeric golgi complex 2
Transcript:
RefSeq NM_007357.2 (NON-CURRENT)
Match location:
Position 2512 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTTCTCTCAGCGTATTT
Hairpin Sequence:
5'-CCGG-GCGTCTTCTCTCAGCGTATTT-CTCGAG-AAATACGCTGAGAGAAGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTTCTCTCAGCGTATTTCTCGAGAAATACGCTGAGAGAAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTTCTCTCAGCGTATTTCTCGAGAAATACGCTGAGAGAAGACGC-3'

Other clones with same target sequence:

(none)