Construct: shRNA TRCN0000343404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001740.2-2339s21c1
DNA Barcode:
GCTAACAGTCAGGGTACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COP1 (64326)
Gene Description:
COP1 E3 ubiquitin ligase
Transcript:
RefSeq NM_001001740.2 (NON-CURRENT)
Match location:
Position 2339 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACAGTCAGGGTACAATT
Hairpin Sequence:
5'-CCGG-GCTAACAGTCAGGGTACAATT-CTCGAG-AATTGTACCCTGACTGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACAGTCAGGGTACAATTCTCGAGAATTGTACCCTGACTGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACAGTCAGGGTACAATTCTCGAGAATTGTACCCTGACTGTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000029721