Construct: shRNA TRCN0000343442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000177.4-750s21c1
DNA Barcode:
CCAACAGCAATCGGTATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSN (2934)
Gene Description:
gelsolin
Transcript:
RefSeq NM_000177.4 (NON-CURRENT)
Match location:
Position 750 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAGCAATCGGTATGAAA
Hairpin Sequence:
5'-CCGG-CCAACAGCAATCGGTATGAAA-CTCGAG-TTTCATACCGATTGCTGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAGCAATCGGTATGAAACTCGAGTTTCATACCGATTGCTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAGCAATCGGTATGAAACTCGAGTTTCATACCGATTGCTGTTGG-3'

Other clones with same target sequence:

  1. TRCN0000029725