Construct: shRNA TRCN0000343484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024560.2-2438s21c1
DNA Barcode:
CCTCCCAAGAAGAACACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSS3 (79611)
Gene Description:
acyl-CoA synthetase short chain family member 3
Transcript:
RefSeq NM_024560.2 (NON-CURRENT)
Match location:
Position 2438 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCAAGAAGAACACTTTA
Hairpin Sequence:
5'-CCGG-CCTCCCAAGAAGAACACTTTA-CTCGAG-TAAAGTGTTCTTCTTGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCAAGAAGAACACTTTACTCGAGTAAAGTGTTCTTCTTGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCAAGAAGAACACTTTACTCGAGTAAAGTGTTCTTCTTGGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000153547