Construct: shRNA TRCN0000343569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016072.4-393s21c1
DNA Barcode:
CCTTATTGGTTGGCCTTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLT1B (51026)
Gene Description:
golgi transport 1B
Transcript:
RefSeq NM_016072.4 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTATTGGTTGGCCTTTGAT
Hairpin Sequence:
5'-CCGG-CCTTATTGGTTGGCCTTTGAT-CTCGAG-ATCAAAGGCCAACCAATAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTATTGGTTGGCCTTTGATCTCGAGATCAAAGGCCAACCAATAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTATTGGTTGGCCTTTGATCTCGAGATCAAAGGCCAACCAATAAGG-3'

Other clones with same target sequence:

  1. TRCN0000162456