Construct: shRNA TRCN0000343619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018052.3-793s21c1
DNA Barcode:
TTGTTGCGAGAGAGGATTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAC14 (55697)
Gene Description:
VAC14 component of PIKFYVE complex
Transcript:
RefSeq NM_018052.3 (NON-CURRENT)
Match location:
Position 793 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTGCGAGAGAGGATTTAC
Hairpin Sequence:
5'-CCGG-TTGTTGCGAGAGAGGATTTAC-CTCGAG-GTAAATCCTCTCTCGCAACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTGCGAGAGAGGATTTACCTCGAGGTAAATCCTCTCTCGCAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTGCGAGAGAGGATTTACCTCGAGGTAAATCCTCTCTCGCAACAA-3'

Other clones with same target sequence:

(none)