Construct: shRNA TRCN0000343626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003081.2-796s21c1
DNA Barcode:
CCAACCAACGTGCAACAAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNAP25 (6616)
Gene Description:
synaptosome associated protein 25
Transcript:
RefSeq NM_003081.2 (NON-CURRENT)
Match location:
Position 796 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACCAACGTGCAACAAAGA
Hairpin Sequence:
5'-CCGG-CCAACCAACGTGCAACAAAGA-CTCGAG-TCTTTGTTGCACGTTGGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACCAACGTGCAACAAAGACTCGAGTCTTTGTTGCACGTTGGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACCAACGTGCAACAAAGACTCGAGTCTTTGTTGCACGTTGGTTGG-3'

Other clones with same target sequence:

  1. TRCN0000156232