Construct: shRNA TRCN0000343662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005998.3-305s21c1
DNA Barcode:
GCTGCCAAGACTATTGCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCT3 (7203)
Gene Description:
chaperonin containing TCP1 subunit 3
Transcript:
RefSeq NM_005998.3 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCAAGACTATTGCAGAT
Hairpin Sequence:
5'-CCGG-GCTGCCAAGACTATTGCAGAT-CTCGAG-ATCTGCAATAGTCTTGGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCAAGACTATTGCAGATCTCGAGATCTGCAATAGTCTTGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCAAGACTATTGCAGATCTCGAGATCTGCAATAGTCTTGGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000157145