Construct: shRNA TRCN0000343681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013327.3-1072s21c1
DNA Barcode:
CGTGGTTAACTTGGACCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARVB (29780)
Gene Description:
parvin beta
Transcript:
RefSeq NM_013327.3 (NON-CURRENT)
Match location:
Position 1072 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGTTAACTTGGACCTCAA
Hairpin Sequence:
5'-CCGG-CGTGGTTAACTTGGACCTCAA-CTCGAG-TTGAGGTCCAAGTTAACCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGTTAACTTGGACCTCAACTCGAGTTGAGGTCCAAGTTAACCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGTTAACTTGGACCTCAACTCGAGTTGAGGTCCAAGTTAACCACG-3'

Other clones with same target sequence:

  1. TRCN0000122960