Construct: shRNA TRCN0000343781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000709.2-1343s21c1
DNA Barcode:
CCCACTGGATCACTTCGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCKDHA (593)
Gene Description:
branched chain keto acid dehydrogenase E1 subunit alpha
Transcript:
RefSeq NM_000709.2 (NON-CURRENT)
Match location:
Position 1343 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTGGATCACTTCGATAA
Hairpin Sequence:
5'-CCGG-CCCACTGGATCACTTCGATAA-CTCGAG-TTATCGAAGTGATCCAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTGGATCACTTCGATAACTCGAGTTATCGAAGTGATCCAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTGGATCACTTCGATAACTCGAGTTATCGAAGTGATCCAGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000028404